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Rabbit Anti-Occludin  antibody (bs-10011R)  
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產品編號 bs-10011R
英文名稱 Rabbit Anti-Occludin  antibody
中文名稱 緊密連接蛋白抗體
別    名 Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin.   
Specific References  (62)     |     bs-10011R has been referenced in 62 publications.
[IF=13.352] Guangdong Bai. et al. Perinatal exposure to glyphosate-based herbicides impairs progeny health and placental angiogenesis by disturbing mitochondrial function. ENVIRON INT. 2022 Dec;170:107579  IF ;  Pig.  
[IF=10.479] Shiyun Chen. et al. Targeted delivery of Chinese herb pair-based berberine/tannin acid self-assemblies for the treatment of ulcerative colitis. J Adv Res. 2021 Nov;:  IF ;  Mouse.  
[IF=10.19] Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119  WB ;  Mouse.  
[IF=9.229] Xiao Wang. et al. Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX  IHC ;  Mouse.  
[IF=8.025] Yao Li. et al. Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity. INT J BIOL MACROMOL. 2022 Aug;215:113  WB ;  Mouse.  
[IF=8.025] Wei Hao. et al. Ginger polysaccharides relieve ulcerative colitis via maintaining intestinal barrier integrity and gut microbiota modulation. INT J BIOL MACROMOL. 2022 Oct;219:730  IF ;  Mouse.  
[IF=7.963] Jun Yan. et al. Intestinal toxicity of micro- and nano-particles of foodborne titanium dioxide in juvenile mice: Disorders of gut microbiota–host co-metabolites and intestinal barrier damage. Sci Total Environ. 2022 May;821:153279  IHC ;  Mouse.  
[IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188  WB ;  Mouse.  
[IF=7.31] Longfei Xiao. et al. Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling Pathway. OXID MED CELL LONGEV. 2023;2023:1708251  WB,IF ;  Pig,Mouse.  
[IF=7.129] Peihao Sun. et al. Lactational exposure to Deoxynivalenol causes mammary gland injury via inducing inflammatory response and impairing blood-milk barrier integrity in mice. ECOTOX ENVIRON SAFE. 2023 Apr;255:114773  IF,WB ;  Mouse.  
[IF=7] Zhang, Yaxin. et al. Stigmasterol attenuates hepatic steatosis in rats by strengthening the intestinal barrier and improving bile acid metabolism. npj Science of Food. 2022 Aug;6(1):1-14  IHC ;  Rat.  
[IF=6.823] Caijun Zhao. et al. Aryl hydrocarbon receptor activation by Lactobacillus reuteri tryptophan metabolism alleviates Escherichia coli-induced mastitis in mice. Plos Pathog. 2021 Jul;17(7):e1009774  IHC ;  Mouse.  
[IF=6.656] Wei Hao. et al. Classical prescription Huanglian Decoction relieves ulcerative colitis via maintaining intestinal barrier integrity and modulating gut microbiota. PHYTOMEDICINE. 2022 Sep;:154468  IF ;  Mouse.  
[IF=6.59] Rongrong Fu. et al. Apigenin remodels the gut microbiota to ameliorate ulcerative colitis.. FRONT NUTR. 2022 Dec;9:1062961-1062961  WB, IHC ;  Mouse.  
[IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965  WB ;  Duck.  
[IF=6.208] Zaozao Pan. et al. Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells. INT J MOL SCI. 2023 Jan;24(8):7242  WB ;  Pig.  
[IF=6.107] Xin Liu. et al. Alteration of gut microbiome and metabolome by Clostridium butyricum can repair the intestinal dysbiosis caused by antibiotics in mice. ISCIENCE. 2023 Feb;:106190  IHC ;  Mouse.  
[IF=6.073] Huang Li. et al. Gut microbiota decreased inflammation induced by chronic unpredictable mild stress through affecting NLRP3 inflammasome. FRONT CELL INFECT MI. 2023 May;13:558  WB ;  Rat.  
[IF=6.064] Irene Palenca. et al. N-Palmitoyl-D-Glucosamine Inhibits TLR-4/NLRP3 and Improves DNBS-Induced Colon Inflammation through a PPAR-α-Dependent Mechanism. BIOMOLECULES. 2022 Aug;12(8):1163  IF ;  Mouse.  
[IF=5.988] Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678  IHC, IF ;  Rat.  
[IF=5.714] Yingxue Li. et al. ProBDNF signaling is involved in periodontitis-induced depression-like behavior in mouse hippocampus. INT IMMUNOPHARMACOL. 2023 Mar;116:109767  WB,IF ;  Mouse.  
[IF=5.714] Mingwei Tong. et al. The Trichinella spiralis-derived antigens alleviate HFD-induced obesity and inflammation in mice. INT IMMUNOPHARMACOL. 2023 Apr;117:109924  IHC ;  Mouse.  
[IF=5.572] Xu Yang. et al. Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc?/GPX4 axis. FOOD CHEM TOXICOL. 2023 May;175:113730  WB ;  Mouse.  
[IF=5.546] Yaqi Chang. et al. Glucagon‐like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model. J Cell Physiol. 2021 Apr;236(4):3015-3032  IHC ;  Pig.  
[IF=5.265] Li, Ke. et al. The Prevention Effect of Lactobacillus plantarum 17–5 on Escherichia coli-Induced Mastitis in Mice. PROBIOTICS ANTIMICRO. 2023 Feb;:1-9  WB ;  Mouse.  
[IF=5.195] Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:116640  WB ;  Rat.  
[IF=4.546] Kexin Wang. et al. Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury. TOXINS. 2022 May;14(5):337  WB ;  Bovine.  
[IF=4.546] Xin-Ran Liu. et al. Radioprotective Effect of Whey Hydrolysate Peptides against γ-Radiation-Induced Oxidative Stress in BALB/c Mice. Nutrients. 2021 Mar;13(3):816  IHC ;  Mouse.  
[IF=4.546] Qing Li. et al. Fumonisin B1 Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins. 2021 Dec;13(12):863  WB ;  Pig.  
[IF=4.432] Juan Zhang. et al. Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora. Eur J Pharmacol. 2022 Feb;916:174730  IHC ;  Mouse.  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 59kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Occludin: 151-250/522 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].

Function:
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Subunit:
Interacts with TJP1/ZO1 and with VAPA.

Subcellular Location:
Membrane; Multi-pass membrane protein. Cell junction, tight junction.

Tissue Specificity:
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts.

Similarity:
Belongs to the ELL/occludin family.
Contains 1 MARVEL domain.

SWISS:
Q16625

Gene ID:
100506658

Database links:

Entrez Gene: 18260 Mouse

Entrez Gene: 100506658 Human

Entrez Gene: 397236 Pig

Entrez Gene: 83497 Rat

Entrez Gene: 403844 Dog

Omim: 602876 Human

SwissProt: Q28269 Dog

SwissProt: Q16625 Human

SwissProt: Q61146 Mouse

SwissProt: Q6P6T5 Rat

Unigene: 592605 Human

Unigene: 4807 Mouse

Unigene: 31429 Rat



產品圖片
Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: Hela (Human) Cell Lysate at 30 ug Primary: Anti-Occludin (bs-10011R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65/55/38/28 kD Observed band size: 62/52/40/25 kD
Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody (bs-10011R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-Occludin antibody (bs-10011R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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