| 產品編號 | bs-9268R |
| 英文名稱 | Rabbit Anti-fbpB antibody |
| 中文名稱 | 結核分枝桿菌Ag85B/fbpB抗體 |
| 別 名 | A85B_MYCTU; Diacylglycerol acyltransferase/mycolyltransferase Ag85B; Mycobacterium tuberculosis Ag85B; M.tuberculosis Ag85b; EC:2.3.1.122; EC:2.3.1.20; DGAT; 30 kDa extracellular protein; Acyl-CoA:diacylglycerol acyltransferase; Antigen 85 complex B (85B; Ag85B); Extracellular alpha-antigen; Fibronectin-binding protein B (Fbps B); MTCY180.32; Rv1886c; |
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Specific References (2) | bs-9268R has been referenced in 2 publications.
[IF=2.738] Liang Wang et al. Clinical Significance of M1/M2 Macrophages and Related Cytokines in Patients with Spinal Tuberculosis. Dis Markers. 2020 May 20;2020:2509454. IHC ; Human.
[IF=1.785] Xiaoqian Shang. et al. Diagnostic value of CXCR3 and its ligands in spinal tuberculosis. Exp Ther Med. 2021 Jan;21(1):1-1 IHC ; Human.
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| 研究領域 | 細胞生物 免疫學 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mycobacteriumtuberculosis |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Ag85B: 231-325/325 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Antigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall. Function: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Subcellular Location: Secreted. Similarity: Belongs to the mycobacterial A85 antigen family. SWISS: P9WQP1 Gene ID: 885785 |
| 產品圖片 |
Sample:
Lane 1: Recombinant M.tuberculosis fbpB protein
Primary: Anti-fbpB (bs-9268R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
