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Rabbit Anti-AMBRA1  antibody (bs-3830R)  
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產品編號 bs-3830R
英文名稱 Rabbit Anti-AMBRA1  antibody
中文名稱 自噬相關基因AMBRA1抗體
別    名 Activating molecule in beclin 1 regulated autophagy; Activating molecule in BECN1 regulated autophagy protein 1; Hypothetical protein FLJ20294; Hypothetical protein LOC55626; KIAA1736; MGC33725; WDR94; AMRA1_HUMAN.  
Specific References  (1)     |     bs-3830R has been referenced in 1 publications.
[IF=2.66] Song, Fuyong, et al. "Involvement of autophagy in tri-ortho-cresyl phosphate-induced delayed neuropathy in hens." Neurochemistry International (2013).  WB ;  
研究領域 腫瘤  心血管  細胞生物  免疫學  發育生物學  神經生物學  細菌及病毒  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Rat (predicted: Human,Mouse,Rabbit,Chicken,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 143kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human AMBRA1: 221-320/1298 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 AMBRA1 regulates autophagy and development of the nervous system. It is a large, previously unknown protein bearing a WD40 domain at its amino terminus, regulates autophagy and has a crucial role in embryogenesis. AMBRA1 is a positive regulator of the Becn1-dependent programme of autophagy, as revealed by its overexpression and by RNA interference experiments invitro. Notably, AMBRA1 functional deficiency in mouse embryos leads to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic cell death.

Function:
Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.

Subunit:
Interacts with BECN1. Probably forms a complex with BECN1 and PIK3C3.

Subcellular Location:
Cytoplasmic vesicle, autophagosome.

Similarity:
Contains 3 WD repeats.

SWISS:
Q9C0C7

Gene ID:
55626

Database links:

Entrez Gene: 55626 Human

Entrez Gene: 228361 Mouse

Omim: 611359 Human

SwissProt: Q9C0C7 Human

SwissProt: A2AH22 Mouse

Unigene: 654644 Human

Unigene: 436667 Mouse



產品圖片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AMBRA1 Polyclonal Antibody, Unconjugated(bs-3830R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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