二抗是與一抗來(lái)源不同的,宿主體內(nèi)制備的,能與一抗或一抗片段結(jié)合的抗體。
二抗可通過(guò)信號(hào)放大提高靈敏度,并為標(biāo)記和檢測(cè)提供了更大的靈活性,通常標(biāo)記酶或熒光素等行使功能。
二抗可以耦聯(lián)不同的標(biāo)記物,如酶(辣根過(guò)氧化酶HRP和堿性磷酸酶AP或其衍生物APAAP,PAP),熒光基團(tuán)(FITC, PE, Cy熒光素, BF熒光素), 生物素和膠體金等。
標(biāo)記二抗具有高特異性、高靈敏度等性質(zhì),被廣泛用于免疫學(xué)實(shí)驗(yàn)中,選用哪種偶聯(lián)物標(biāo)記的二抗取決于具體的實(shí)驗(yàn)。
博奧森熱銷二抗,標(biāo)記二抗:
山羊抗兔 IgG: HRP | FITC | BF488 | BF594 | BF555 | IRDye® 800CW | Biotin | Cy3
山羊抗小鼠 IgG: HRP | FITC | BF488 | BF594
兔抗雞 IgG: FITC 兔抗山羊 IgG: HRP 兔抗豬 IgG: HRP
靶標(biāo)物種
二抗名稱
未標(biāo)記
產(chǎn)品應(yīng)用
標(biāo)記類型
Rabbit
Donkey anti-Rabbit IgG
bs-0295D
WB, IHC-P, IHC-F, ELISA
AbBy Fluor® 350
AbBy Fluor® 405
AbBy Fluor® 488
AbBy Fluor® 555
AbBy Fluor® 594
AbBy Fluor® 647
AbBy Fluor® 680
AbBy Fluor® 750
HRP
FITC
Biotin
RBITC
PE
PE-Cy5
PE-Cy5.5
PE-Cy7
APC
APC-Cy5.5
APC-Cy7
Cy3
Cy5
Cy5.5
Cy7
PerCP
PerCP-Cy5.5
IRDye 800CW
AP/ALP
Gold/Co-Au
ATTO550
AE
SA
Donkey anti-Rabbit IgG whole serum
bs-0295Ds
WB, IHC-P, IHC-F, ELISA
Goat anti-Rabbit IgG
bs-0295G
-
Goat anti-Rabbit IgG whole serum
bs-0295Gs
WB, IHC-P, IHC-F, ELISA
AffiniPure F(ab')? Fragment Goat anti-Rabbit IgG (H+L)
bs-60295G
-
Mouse anti-Rabbit IgG (H + L)
bs-0295M
ELISA
Mouse anti-Rabbit IgG ascites
bs-0295Ms
WB, IHC-P, IHC-F, ELISA
Mouse anti-Rabbit IgG mAb
bsm-0295M
WB, IHC-P, IHC-F, ICC, IF, ELISA
Mouse anti-Rabbit IgM
bs-0369M
IHC-P, IHC-F, ELISA
Rabbit anti-Avidin
bs-0312R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Avidin whole serum
bs-0312Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Biotin
bs-0311R
IHC-P, IHC-F, ELISA
Rabbit anti-BSA whole serum
bs-0292Rs
-
Rabbit anti-digoxin whole serum
bs-0356Rs
-
Mouse
Goat anti-Mouse IgG
bs-0296G
-
Goat anti-Mouse IgG whole serum
bs-0296Gs
WB, IHC-P, IHC-F, ELISA
AffiniPure F(ab')? Fragment Goat anti-Mouse IgG (H+L)
bs-60296G
-
Goat anti-Mouse IgM
bs-0368G
WB, IHC-P, IHC-F, ELISA
Goat anti-Mouse IgM whole serum
bs-0368Gs
WB, IHC-P, IHC-F, IF, ELISA
Rabbit anti-Mouse IgG
bs-0296R
-
Rabbit anti-Mouse IgG whole serum
bs-0296Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Mouse IgG-Fc
bs-0377R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Mouse IgG Fc whole serum
bs-0377Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Mouse Kappa light chain
bs-0330R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Mouse Kappa Chain whole serum
bs-0330Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Mouse IgM
bs-0368R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Mouse IgM whole serum
bs-0368Rs
WB, IHC-P, IHC-F, ELISA
Rat anti-Mouse IgD
bsm-34233A
Flow-Cyt
Human
Human IgG-Fc
bsm-0365M
WB, IHC-P, IHC-F, ICC, IF, ELISA
Donkey anti-Human IgG
bs-0297D
WB, IHC-P, IHC-F, ELISA
Donkey anti-Human IgG whole serum
bs-0297Ds
WB, IHC-P, IHC-F, ELISA
Goat anti-Human IgG
bs-0297G
-
Goat anti-Human IgG whole serum
bs-0297Gs
WB, IHC-P, IHC-F, ELISA
Goat anti-Human IgM
bs-0345G
WB, IHC-P, IHC-F, ELISA
Goat anti-Human IgM whole serum
bs-0345Gs
WB, IHC-P, IHC-F, ELISA
Goat anti Human IgE
bs-2226G
-
Goat anti-Human IgA whole serum
bs-0360Gs
-
Mouse anti-Human IgG
bs-0297M
ELISA
Mouse anti-Human IgG ascites
bs-0297Ms
WB, IHC-P, IHC-F, ELISA
Mouse anti-Human IgG mAb
bsm-41525M
ELISA
Mouse anti-Human IgG monoclonal antibody
bsm-0297M
WB, IHC-P, IHC-F, ELISA
Mouse anti-Human IgM
bs-0345M
WB, IHC-P, IHC-F, ELISA
Mouse anti-Human IgM
bsm-41524M
ELISA
Mouse anti-Human IgM ascites
bs-0345Ms
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Human IgG
bs-0297R
-
Rabbit anti-Human IgG whole serum
bs-0297Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Human IgG / Streptavidin
bsa-0297R
-
Rabbit anti-Human IgG F (ab')2
bs-0364R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Human IgG F(ab')? whole serum
bs-0364Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Human IgG Fc
bs-0365R
WB, IHC-P, IHC-F, ICC, IF, ELISA
Rabbit anti-Human IgG Fc whole serum
bs-0365Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Human IgG heavy chain constant region whole serum
bs-0411Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Human IgM
bs-0345R
WB, IHC-P, IHC-F
Rabbit anti-Human IgM whole serum
bs-0345Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Human IgA
bs-0360R
ELISA
Rabbit anti-Human IgA whole serum
bs-0360Rs
-
Rat
Goat anti-Rat IgG
bs-0293G
-
Goat anti-Rat IgG whole serum
bs-0293Gs
WB, IHC-P, IHC-F, ELISA
Mouse anti-Rat IgG
bs-0293M
WB, IHC-P, IHC-F, ELISA
Mouse anti-Rat IgG ascites
bs-0293Ms
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Rat IgG
bs-0293R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Rat IgG whole serum
bs-0293Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Rat IgM
bs-0346R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Rat IgM whole serum
bs-0346Rs
WB, IHC-P, IHC-F, ELISA
Goat
Donkey anti-Goat IgG
bs-0294D
WB, IHC-P, IHC-F, ELISA
Donkey anti-Goat IgG whole serum
bs-0294Ds
WB, IHC-P, IHC-F, ELISA
Mouse anti-Goat IgG
bs-0294M
-
Mouse anti-Goat IgG ascites
bs-0294Ms
WB, IHC-P, IHC-F, ELISA
Mouse anti Goat IgG monoclonal antibody
bsm-0294M
WB, IHC-P, IHC-F, ICC, IF, ELISA
Mouse anti-Goat IgM ascites
bs-0370Ms
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Goat IgG
bs-0294R
-
Rabbit anti-Goat IgG whole serum
bs-0294Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Goat IgM
bs-0370R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Goat IgM whole serum
bs-0370Rs
WB, IHC-P, IHC-F, ELISA
Bovine
Goat anti-Bovine IgG
bs-0326G
WB, IHC-P, IHC-F, ELISA
Goat anti-Bovine IgG whole serum
bs-0326Gs
WB, IHC-P, IHC-F, ELISA
Mouse anti-Bov IgG
bs-0326M
WB, IHC-P, IHC-F, ELISA
Mouse anti-Bovine IgG ascites
bs-0326Ms
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Bov IgG
bs-0326R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Bovine IgG whole serum
bs-0326Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Bovine IgM
bs-0327R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Bovine IgM whole serum
bs-0327Rs
WB, IHC-P, IHC-F, ELISA
Cat
Rabbit anti-Cat IgG H&L
bs-10749R
IHC-P, IHC-F, ELISA
Chicken
Goat anti-Chicken IgG
bs-0310G
WB, IHC-P, IHC-F, ELISA
Goat anti-Chicken IgG whole serum
bs-0310Gs
WB, IHC-P, IHC-F, ELISA
Goat anti-Chicken IgY
bs-0432G
WB, ELISA
Mouse anti-Chicken IgG H&L
bs-0310M
-
Mouse anti-Chicken IgY
bs-0432M
WB, IHC-P, IHC-F, ICC, IF, ELISA
Rabbit anti-Chicken IgG
bs-0310R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Chicken IgG whole serum
bs-0310Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Chicken IgM
bs-0314R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Chicken IgM whole serum
bs-0314Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Chicken IgY
bs-0432R
WB, IHC-P, IHC-F, IF, ELISA
Dog
Rabbit anti-Dog IgG
bs-0303R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Dog IgG whole serum
bs-0303Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Dog IgM
bs-0373R
IHC-P, IHC-F, IF, ELISA
Guinea pig
Donkey anti-Guinea pig IgG
bs-0358D
WB, IHC-P, IHC-F, ELISA
Donkey anti-Gpig IgG whole serum
bs-0358Ds
WB, IHC-P, IHC-F, ELISA
Goat anti-Guinea pig IgG
bs-0358G
WB, IHC-P, IHC-F, ELISA
Goat anti-Gpig IgG whole serum
bs-0358Gs
WB, IHC-P, IHC-F, ELISA
Mouse anti-Guinea pig IgG
bs-0358M
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Guinea pig IgG
bs-0358R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Guinea pig IgG whole serum
bs-0358Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Guinea pig IgM
bs-0375R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Guinea pig IgM whole serum
bs-0375Rs
WB, IHC-P, IHC-F, ELISA
Horse
Rabbit anti-Horse IgG
bs-0308R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Horse IgG whole serum
bs-0308Rs
WB, IHC-P, IHC-F, ELISA
Mongolian Gerbil
Rabbit anti-MG IgG
bs-0403R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-MG IgG whole serum
bs-0403Rs
WB, IHC-P, IHC-F, ELISA
Mink
Rabbit anti-Mink IgG
bs-0777R
WB, IHC-P, IHC-F, ELISA
Monkey
Rabbit anti-Monkey IgG
bs-0335R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Monkey IgG whole serum
bs-0335Rs
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Monkey IgM
bs-0336R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Monkey IgM whole serum
bs-0336Rs
WB, IHC-P, IHC-F, ELISA
Pig
Rabbit anti-Pig IgG
bs-0309R
WB, IHC-P, IHC-F, ELISA
Rabbit anti-Pig IgG whole serum
bs-0309Rs
WB, IHC-P, IHC-F, ELISA
標(biāo)記二抗高分文獻(xiàn)展示